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| 96-Well Plasmid Kit |
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The 96-Well Plasmid Kit was designed for high-throughput plasmid DNA purification from 1-2 ml of cultured bacterial cells. Modified Alkaline Lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of each well of the plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 1 hour. |
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Specifications
- Sample: 1-2 ml of cultured bacterial cells
- Format: 96-Well plates
- Yield: 5-10 μg (high-copy plasmid), 0.5-5 μg (low-copy plasmid)
- Operation: centrifuge/vacuum manifold
- Applications: Fluorescent/Radioactive Sequencing, Restriction Enzyme Digestion, Library
Screening, Ligation, Transformation and PCR
- Storage: room temperature for up to 2 years
- Operation time: within 1 hour
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Test Data
3 µl from a 100 µl elution product was loaded in each lane.
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Sequencing Data
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Sequencing of plasmid DNA purified with Geneaid’s 96-Well Plasmid Kit
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Order Information
| High-Speed Plasmid Mini Kit |
PD100/300 |
| High-Speed Plasmid Mini Kit (10-50 Kb) |
PDL100/300 |
| High-Speed Plasmid Advance Kit (50-100 ml) |
PA025 |
| Geneaid Plasmid Midi Kit |
PI025 |
| Geneaid Plasmid Midi Kit (Endotoxin Free) |
PIE25 |
| Geneaid Plasmid Maxi Kit |
PM010/25 |
| Geneaid Plasmid Maxi Kit (Endotoxin Free) |
PME10/25 |
| 96-Well Plasmid Kit |
PDA04/10 |
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