|
|
|
|
|
|
| |
High-Speed Plasmid Advance Kit
(50-100 ml bacteria) |
| |
The High-Speed Plasmid Advance Kit was designed for rapid purification of plasmid or cosmid DNA from 50-100 ml of cultured bacterial cells. Modified Alkaline Lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE. Typical yields are 200-350 μg for high-copy number plasmid or 30-100 μg for low-copy number plasmid from 50 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 1 hour. |
| |
 |
| |
|
|
|
|
Specifications
- Sample: 50-100 ml of bacterial cells
- Format: spin column (centrifuge)
- Yield: up to 500 μg of plasmid/cosmid DNA
- Applications: Restriction Enzyme Digestion,
Library Screening, Ligation, PCR,
Transformation/Sequencing Reactions
- Operation time: within 1 hour
- Storage: room temperature for up to 2 years
Test Data
1 2 3 4
Lane 1:1 Kb DNA Ladder
Lane 2-4: pBluescript II
Order Information
| High-Speed Plasmid Mini Kit |
PD100/300 |
| High-Speed Plasmid Mini Kit (10-50 Kb) |
PDL100/300 |
| High-Speed Plasmid Advance Kit (50-100 ml) |
PA025 |
| Geneaid Plasmid Midi Kit |
PI025 |
| Geneaid Plasmid Midi Kit (Endotoxin Free) |
PIE25 |
| Geneaid Plasmid Maxi Kit |
PM010/25 |
| Geneaid Plasmid Maxi Kit (Endotoxin Free) |
PME10/25 |
| 96-Well Plasmid Kit |
PDA04/10 |
|
Quick Protocol
.png)
|
| |
|
|
|
 |
|
|
|
