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| Plasmid Midiprep Kit |
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Plasmid Midiprep Kits use pre-packed anion-exchange resin columns to purify plasmid DNA from 50-100 ml of cultured bacterial cells. Modified Alkaline Lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the column and the contaminants are removed with the PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 2 hours without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for Transfection, Sequencing Reactions, Ligation, PCR, and In-vitro Transcription, Microinjection, Restriction Enzyme Digestion and Gene Gun. |
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Specifications
- Sample: 50-100 ml of cultured bacterial cells
- Format: anion-exchange resin column
- Yield: up to 250 µg
- Purity: = 2 x CsCl – gradient centrifugation
- Applications: Transfection, Sequencing,
Ligation, PCR, In-vitro Transcription, Microinjection, Restriction Enzyme Digestion,
Gene Gun
- Storage: room temperature for up to 2 years
- Operation time: within 2 hours
High-Copy/Low-Copy Plasmid Test Data
M C F W E M C F W E
Plasmid pBluescript (high-copy) and pBR322 (low-copy) were
purified with the Plasmid Midiprep Kit. DNA in crude lysate
from Alkaline Lysis and fractions from each gravity-flow step
were collected by Isopropanol precipitation and loaded into
each lane.
M: 1 Kb DNA Ladder, C: crude lysate, F: flow-through,
W: wash, E: elution
Order Information
| High-Speed Plasmid Mini Kit |
PD100/300 |
| High-Speed Plasmid Mini Kit (10-50 Kb) |
PDL100/300 |
| High-Speed Plasmid Advance Kit (50-100 ml) |
PA025 |
| Geneaid Plasmid Midi Kit |
PI025 |
| Geneaid Plasmid Midi Kit (Endotoxin Free) |
PIE25 |
| Geneaid Plasmid Maxi Kit |
PM010/25 |
| Geneaid Plasmid Maxi Kit (Endotoxin Free) |
PME10/25 |
| 96-Well Plasmid Kit |
PDA04/10 |
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Quick Protocol |
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