The Total RNA Miniprep Kit (Blood/Cultured Cell) was designed specifically for total RNA purification from fresh whole human blood and cultured cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase (optional DNase treatments can be followed to remove unwanted DNA residue). RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column and once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free water.
