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| Total RNA Mini Kit (Tissue) |
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The Total RNA Miniprep Kit (Tissue) was designed specifically for total RNA purification from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free water. Phenol extraction or alcohol precipitation is not required. |
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Specifications
- Sample: up to 25 mg of tissue
up to 25 mg of paraffin-embedded tissue
- Format: spin column (centrifuge)
- Yield: 5-30 μg
- Applications: RT-PCR, Northern Blotting
Primer Extension, mRNA Selection
cDNA Synthesis, RNase Protection Assay
- Operation time: within 30 minutes
- Storage: room temperature for up to 2 years
Test Data Coming Soon!
Order Information
| Total RNA Mini Kit (Blood/Cultured Cell) |
RB050/100/300 |
| Total RNA Mini Kit (Tissue) |
RT050/100/300 |
| Total RNA Mini Kit (Plant) |
RP050/100/300 |
| Total RNA Maxi Kit (Blood/Cultured Cell) |
RBM10/25 |
| Total RNA Maxi Kit (Tissue) |
RTM10/25 |
| Total RNA Maxi Kit (Plant) |
RPM10/25 |
| 96-Well Total RNA Kit |
RBP04/10 |
| miRNA Isolation Kit |
PU009100 |
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Quick Protocol
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