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| Total RNA Maxi Kit (Blood/Cultured Cell) |
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The Total RNA Purification Maxi Kit (Blood/Cultured Cell) was designed specifically for total RNA purification from fresh whole human blood and cultured cells. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. The RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free water. The entire procedure can be completed within 1 hour and the purified RNA is ready for use in RT-PCR, Northern Blotting, Primer Extension and cDNA Library Construction.
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Specifications
- Sample: up to 5 ml of whole blood
cultured cells (up to 1 x 108)
bacterial cells (up to 1 x 1010)
- Format: spin column (centrifuge)
- Yield: 50-300 μg
- Operation time: within 1 hour
- Applications: RT-PCR, Northern Blotting
Primer Extension, mRNA Selection
cDNA Synthesis
Test Data Coming Soon!
Order Information
| Total RNA Mini Kit (Blood/Cultured Cell) |
RB050/100/300 |
| Total RNA Mini Kit (Tissue) |
RT050/100/300 |
| Total RNA Mini Kit (Plant) |
RP050/100/300 |
| Total RNA Maxi Kit (Blood/Cultured Cell) |
RBM10/25 |
| Total RNA Maxi Kit (Tissue) |
RTM10/25 |
| Total RNA Maxi Kit (Plant) |
RPM10/25 |
| 96-Well Total RNA Kit |
RBP04/10 |
| miRNA Isolation Kit |
PU009100 |
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Quick Protocol
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