Geneaid 旭基: Gel Extraction Kit, PCR Cleanup Kit, Genomic DNA Purification Kit, Plasmid Miniprep Kit, DNA Mini Kit, RNA Mini Kit

Sitemap

│

Magnetic Bead Systems

Enzymes

Protein

Other

Viral DNA/RNA

Home > products > Viral DNA/RNA > Viral Nucleic Acid Extraction Kit II

Viral Nucleic Acid Extraction Kit II

The Viral Nucleic Acid Extraction Kit II was designed specifically for viral DNA/RNA purification from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures (H1N1, HIV/AIDS, HPV, HBV, etc.). DNA/RNA viruses are lysed quickly and efficiently using the lysis buffer which is a highly concentrated solution of chaotropic salt. When combined with ethanol, the AD Buffer creates optimum conditions for nucleic acid binding to the glass fiber matrix of the column. Contaminants, such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash step. nucleic acid is eluted in RNase-free water and is then ready for use in subsequent reactions.

Specifications

Sample: 200 µl samples of plasma,
serum, body fluids,
supernatant of viral infected cell cultures
Format: spin column (centrifuge)
Operation time: within 40 minutes
Applications: RT-PCR/PCR,
Real-time PCR, Real-time RT-PCR,
Automated Fluorescent DNA
Sequencing
Storage: room temperature for up to 2 years

Order Information

Viral Nucleic Acid Extraction Kit II (200 µl)	VR050/100/300
Viral Nucleic Acid Extraction Kit III (1 ml)	VI050/100/300
96-Well Viral DNA/RNA Extraction Kit	VNP04/10

Test Data

HAV RT-PCR

M   1    2   3   M   4   5    6

Geneaid
M: Marker
1: Extracted HAV from original serum
2: Extracted HAV from 10X diluted serum
3: Extracted HAV from 100X diluted serum

Competitor Q
M: Marker
4: Extracted HAV from original serum
5: Extracted HAV from 10X diluted serum
6: Extracted HAV from 100X diluted serum

HBV Real-time PCR (Copies/ml)

	Original Serum	10X Diluted Serum	100X Diluted Serum
Geneaid	2.17 × 10⁷	4.47 × 10⁶	8.48 × 10⁵
Q	2.36 × 10⁷	4.94 × 10⁶	1.67 × 10⁶

One-step Real-time Qauntitative RT-PCR

Human beta-globin mRNA was detected in total RNA human placenta samples ranging from 3 µg (E7 copies) to 0.3 pg (1 copy) using the ABI 7300 Sequence Detection.

Quick Protocol

Dynamic Range, Sensitivity, And Standard Curve of the Viral Nucleic Acid Extraction Kit

Quantitation of Viral DNA/RNA Purified Using the Viral Nucleic Acid Extraction Kit

Real-time qPCR and 1-step qRT-PCR reactions were conducted to evaluate the performance of Geneaid’s Viral Nucleic
Acid Extraction Kit. HBV (DNA), HCV (RNA), HIV (RNA), and HTLV (RNA) were purified from 200 µl of positive clinical
serum samples. The reactions were conducted using the ABI 7300 Sequence Detection System. Blood samples
containing various amounts of DNA/RNA viruses ranging from 1 x E2 to 1 x E6 copies/ml were successfully detected
and identified.