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Home > products > Viral DNA/RNA > Viral Nucleic Acid Extraction Kit III

Viral Nucleic Acid Extraction Kit III

The Viral Nucleic Acid Extraction Kit III was designed specifically for simultaneous virus DNA/RNA purification from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures (H1N1, HIV/AIDS, HPV, HBV, etc.). The method is comprised of concentrating viral particles from serum, lysis of viral particles, viral DNA/RNA binding to the surface of the glass fiber membrane, and releasing of DNA/RNA into the release buffer. 10¹-10⁹ copies of viral DNA/RNA can be purified from 1 ml of serum within 1 hour. The purified viral DNA/RNA can be used directly in qPCR and qRT-PCR assays.

Specifications

Sample: 1 ml of serum or plasma
Format: spin column (centrifuge)
Operation time: within 40 minutes
Applications: RT-PCR/PCR,
Real-time PCR, Real-time RT-PCR,
Automated Fluorescent DNA Sequencing
Storage: room temperature for up to 2 years

Order Information

Viral Nucleic Acid Extraction Kit II (200 µl)	VR050/100/300
Viral Nucleic Acid Extraction Kit III (1 ml)	VI050/100/300
96-Well Viral DNA/RNA Extraction Kit	VNP04/10

Test Data

One-step Real-time Quantitative RT-PCR

Human beta-globin mRNA was detected in total RNA human placenta
samples ranging from 3 µg (E7 copies) to 0.3 pg (1 copy) using
ABI 7300 Sequence Detection.

Quick Protocol

Dynamic Range, Sensitivity, And Standard Curve of the Viral Nucleic Acid Extraction Kit

Quantitation of Viral DNA/RNA Purified Using the Viral Nucleic Acid Extraction Kit

Real-time qPCR and 1-step qRT-PCR reactions were conducted to evaluate the performance of Geneaid’s Viral Nucleic Acid Extraction Kit. HBV (DNA), HCV (RNA), HIV (RNA), and HTLV (RNA) were purified from 200 µl of positive clinical serum samples. The reactions were conducted using the ABI 7300 Sequence Detection System. Blood samples containing various amounts of DNA/RNA viruses ranging from 1 x E2 to 1 x E6 copies/ml were successfully detected and identified.