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  Viral DNA/RNA
Home > products > Viral DNA/RNA > 96-Well Viral DNA/RNA Extraction Kit
 
 
96-Well Viral DNA/RNA Extraction Kit
 

The 96-Well Viral DNA/RNA Extraction Kit was designed specifically for high-throughput virus DNA/RNA purification of from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures (H1N1, HIV/AIDS, HPV, HBV, etc.). DNA/RNA viruses are lysed quickly and efficiently when exposed to the lysis buffer which is a highly concentrated solution of chaotropic salt. When combined with ethanol, the AD Buffer creates optimum conditions for nucleic acid binding to the glass fiber matrix of the Viral DNA/RNA Binding Plate. Contaminants such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash step. The nucleic acid is eluted in RNase-free water and is then ready for use in subsequent reactions including, Real-time PCR/RT-PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. The detection limit for certain viruses depends on the sensitivity of individual PCR or RT-PCR assay.

 
 
 
Specifications
  • Sample: 200 µl samples of plasma, serum, body fluids,

    supernatant of viral infected cell cultures

  • Yield: up to 30 µg/well
  • Format: vacuum manifold
  • Operation time: within 40 minutes
  • Applications: RT-PCR/PCR, Real-time PCR/Real-time

    RT-PCR, Automated Fluorescent DNA Sequencing

  • Storage: room temperature for up to 2 years 
     

 

Test Data


The 48 templates subjected to PCR were purified using the 96-Well
Viral DNA/RNA Extraction Kit. The signals were visualized on an
EtBr stained agarose gel.

 


 Recovery Rate of 96-Well Viral DNA/RNA Extraction Kit



 200 µl of serum from each of the 96 clinical blood samples was  
 subjected to the kit for viral DNA/RNA purification. The purified 
 viral DNA/RNA was recovered in 50 µl of DNase and
 RNase-free water. The final volume from each well was
 determined and the results were plotted against corresponding
 wells.


Order Information

Viral Nucleic Acid Extraction Kit II (200 µl) VR050/100/300
Viral Nucleic Acid Extraction Kit III (1 ml) VI050/100/300
96-Well Viral DNA/RNA Extraction Kit VNP04/10

 
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