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SYNCstore™ STM (SS1000)

Covid-19 Solutions/Sample Storage Buffer

The SYNCstore™ STM is a specimen transport medium and designed for sample collection, shipping, and storage, especially suitable for nasopharyngeal/ oropharyngeal swabs. In addition, the SYNCstore™ STM can be used for virus/bacteria inactivation and DNA/RNA stabilization. Samples in SYNCstore™ STM stored at 22-27℃ is stable for 1 week and at -20℃ or -70℃ is stable for months to 1 year. The SYNCstore™ STM is compatible with most of nucleic acid extraction kits, including column based and magnetic beads based kits. The purified DNA/RNA from SYNCstore™ STM is suitable for subsequent nucleic acid tests (NAT) such as qPCR and qRT-PCR.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # SS1000)

  • Format: 1 ml/ 3 ml SYNCstoreTM STM in 7 ml tube with cap. With/ without nasopharyngeal swabs.
  • Sample: Sputum, saliva, nasopharyngeal/ oropharyngeal swabs, nasal washes, urine, fecal.
  • Features: Virus/bacteria killing 
                        Nucleases inactivation 
                        RNA stabilization
                        Sample long term storage 
                        Room temperature transport
                        Human cfDNA/cfRNA stabilization

APPLICATIONS

sample collection, shipping, storage. Virus/bacteria inactivation, DNA/RNA stabilization.

 

SYNCstoreTM STM Test Data

Bacteria killing

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Test 1 .Staphylococcus aureus (Gram-positive) were grown to 0.8 of OD600. A 10,000x dilution was made, same amount cells with SYNCstoreTM STM treatment at room temperature for 1 hour and without treatment were plated on BAP plates and incubated for 48 hrs. The results showed that the Staphylococcus aureus cells can be completely killed by SYNCstoreTM STM.

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Test 2 .Escherichia coli (Gram-negative) were grown to 0.8 of OD600. A 10,000x dilution was made, same amount cells with SYNCstoreTM STM treatment at room temperature for 1 hour and without treatment were plated on LB plates and incubated for 18 hrs. The results showed that the Escherichia coli cells can be completely killed by SYNCstoreTM STM.

Virus killing

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The MS2 phage (RNA, non-enveloped, icosohedral virus) with SYNCstoreTM STM treatment and without treatment were mixed with the cells and pour to a plate with a lawn of actively growing compatible bacteria. After overnight growth, the plaques can be visualized, quantitated, and then titer determined. The results showed that the MS2 phage can be completely killed by SYNCstoreTM STM.

RNA stability

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SYNCstoreTM STM provides long-term storage of Escherichia coli total RNA. It efficiently protects all RNA species (23S RNA, 16S, RNA, mRNA, and 5S RNA/tRNA from nucleases attacking. Lane 1: Original RNA. Lane 2: RNA stored in the SYNCstoreTM STM medium for 1 day at room temperature. Lane 3: RNA stored in the SYNCstoreTM STM medium for 2 days at room temperature. Lane 4: RNA stored in the SYNCstoreTM STM medium for 3 days at room temperature. Lane 5: RNA stored in the SYNCstoreTM STM medium for 7 days at room temperature.

Compatible to spin column and magnetic bead nucleic acid purification kits

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Different copy numbers of nucleic acid from M13 DNA phage, MS2 RNA phage, long synthetic oligo DNA, HBV DNA virus, HCV RNA virus, and human genomic DNA were spiked into SYNCstoreTM STM. The nucleic acid were then purified from SYNCstoreTM STM medium using spin column and magnetic beads format kits individually. The nucleic acid recovery were determined by qPCR or qRT-PCR.

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