Urine DNA Extraction Kit (UR050, UR100)
Urine DNA Extraction Kit (UR050, UR100)
The Presto™ Urine DNA Extraction Kit is designed for rapid isolation of high-quality cellular and cell-free DNA from up to 5 ml of urine. The kit includes uniquely designed Column Extension Tubes which allow for increased sample volume and yield. Urine samples are lysed using Proteinase K and a buffer containing chaotropic salt. The lysate is mixed with a binding buffer to facilitate DNA binding to the column membrane. The column is then washed and DNA is eluted with Elution Buffer. The entire procedure can be completed within 60 minutes and the purified DNA is ready for use in a variety of downstream applications such as qPCR, Next-Generation sequencing and DNA methylation analysis. 

Specifications

  • Operation time: Purify cellular and cell-free DNA within 60 minutes.
  • Sample: 1-5 ml of urine
  • Format:  Spin columns combined with column extension tubes using vacuum or centrifuge
  • Yield: 1-20 ng of DNA per ml of male urine. 5-1000 ng of DNA per ml of female urine.
  • Elution Volume: 30-50 µl
  • Storage: dry at room temperature (20~25ºC)

Applications

qPCR, Next-Generation sequencing and DNA methylation analysis

Caution

During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.

Components

  • UR1 Buffer
  • UR2 Buffer
  • Proteinase K
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer
  • Carrier RNA
  • Column Extender
  • GD Column
  • 2 ml Collection Tube

Quality Control

The quality of the Presto Urine Extraction Kit is tested on a lot-to-lot basis by isolating DNA from 2 ml of urine. Following the purification process, the purified DNA integrity was assessed by qPCR.
 
Presto™ Urine DNA Extraction Kit Functional Test Data
Urine kit 01

Figure 1. Cellular and cell-free DNA was effectively purified from 5 ml of healthy female urine using Presto™ Urine DNA Extraction Kit. 

The purified DNA was eluted with 30 µl Elution Buffer. Purified DNA was analyzed using the Qsep100 capillary electrophoresis system (Bioptic).

Urine kit02

Figure 2Total genomic DNA was isolated from 2, 5 ml of female urine samples (2 replicates of each sample) using PrestoTM Urine DNA Extraction Kit. 

The purified DNA was eluted with 30 μl Elution Buffer. A real-time PCR assay was then performed with 1 μl of purified DNA as template, primers (designed to amplify the human GAPDH gene), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The red line corresponds to the purified DNA from 5 ml of urine samples, the green line corresponds to the purified DNA from 2 ml of urine samples. The black line corresponds to the No Template Control.

 

Genomic DNA Extraction Kits Specifications

Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
N/A
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 30-200 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes

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GT050/100/300
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GP100
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GMB100/300
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GSK100/300
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GBYB100/300
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